Identifikasi Gen Hemoglobin A (HbA) pada Pasien Gagal Ginjal Kronik (GGK) di Rumah Sakit Santa Elisabeth Medan Tahun 2026

Ruth Angelika Natasya Nadeak; Paska Ramawati Situmorang; Ruth Agree Kartini Sihombing; Seri Rayani Bangun

doi:10.71456/jik.v5i1.2706

Authors

Ruth Angelika Natasya Nadeak Program Studi Sarjana Terapan Teknologi Laboratorium Medik, Sekolah Tinggi Ilmu Kesehatan Santa Elisabeth Medan
Paska Ramawati Situmorang Program Studi Sarjana Terapan Teknologi Laboratorium Medik, Sekolah Tinggi Ilmu Kesehatan Santa Elisabeth Medan
Ruth Agree Kartini Sihombing Program Studi Sarjana Terapan Teknologi Laboratorium Medik, Sekolah Tinggi Ilmu Kesehatan Santa Elisabeth Medan
Seri Rayani Bangun Program Studi Sarjana Terapan Teknologi Laboratorium Medik, Sekolah Tinggi Ilmu Kesehatan Santa Elisabeth Medan

DOI:

https://doi.org/10.71456/jik.v5i1.2706

Keywords:

Chronic Kidney Disease, Electrophoresis, Hemoglobin A, Hemoglobin Level, Polymerase Chain Reaction

Abstract

: Gagal Ginjal Kronik (GGK) merupakan kondisi yang dapat disertai penurunan kadar hemoglobin akibat gangguan fungsi ginjal, sedangkan identifikasi gen Hemoglobin A (HbA) diperlukan untuk mengetahui keberadaan fragmen DNA target. Penelitian ini bertujuan mengidentifikasi gen HbA pada pasien GGK di Rumah Sakit Santa Elisabeth Medan Tahun 2026. Penelitian menggunakan desain deskriptif analitik dengan pemeriksaan laboratorik melalui isolasi DNA, Polymerase Chain Reaction (PCR), dan elektroforesis gel agarosa. Populasi penelitian adalah pasien GGK yang mengalami anemia dan menjalani hemodialisis, dengan sampel sebanyak 20 pasien menggunakan teknik total sampling. Instrumen meliputi peralatan isolasi DNA, PCR, elektroforesis, dan gel documentation. Data dianalisis secara deskriptif menggunakan IBM SPSS 26 berdasarkan kadar Hb dan visualisasi pita DNA. Hasil penelitian menunjukkan rata-rata kadar Hb sebesar 8,9 g/dL dengan rentang 5,7 hingga 10,8 g/dL. Elektroforesis menunjukkan pita DNA gen HbA pada ukuran sekitar 171 bp, meskipun beberapa pita tampak samar. Kesimpulan penelitian menunjukkan bahwa gen HbA berhasil teridentifikasi pada sampel pasien GGK, sedangkan kadar Hb seluruh responden berada di bawah nilai normal.

References

Abubakar, R. (2019). Pengantar metodologi penelitian. Suka-Press UIN Sunan Kalijaga.

Adiputra, M. S. (2021). Metodologi penelitian kesehatan. Yayasan Kita Menulis.

Anggreni, D. (2022). Buku ajar metodologi penelitian kesehatan. Penerbit STIKes Majapahit Mojokerto.

Bila, S. (2025). Hubungan antara anemia dan fungsi ginjal pada pasien dengan penyakit ginjal kronis. Scientific Journal, 4(1), 6–14. https://doi.org/10.56260/sciena.v4i1.186

Debode, F. (2017). The influence of amplicon length on real-time PCR results. Biotechnology, Agronomy, Society and Environment (BASE), 21(1), 3–11. https://doi.org/10.25518/1780-4507.13461

Eviwindha, & Suara, D. R. (2024). Karakteristik faktor risiko pasien chronic kidney disease (CKD) yang menjalani hemodialisa. Jurnal Media Akademik (MAK), 8(2), 59–63. https://doi.org/10.33655/mak.v8i1.194

Gnitecki, S. (2024). Anemia of chronic kidney disease: A narrative review of its pathophysiology and management. Biomedicines, 12(6), Article 1191. https://doi.org/10.3390/biomedicines12061191

Hadju, V., & Indrawati, E. S. (2025). Anemia: Etiology, pathophysiology, impact, and prevention: A review. Iranian Journal of Public Health, 54(3), 509–520. http://ijph.tums.ac.ir

Hanna, R. (2021). Burden of anemia in chronic kidney disease: Beyond erythropoietin. Advances in Therapy, 38(1), 52–75. https://doi.org/10.1007/s12325-020-01524-6

Harteveld, C. L. (2022). The hemoglobinopathies, molecular disease mechanisms and diagnostics. International Journal of Laboratory Hematology, 44(Suppl. 1), 28–36. https://doi.org/10.1111/ijlh.13885

Hasibuan, S. F. (2024). Profile of anemia and the relationship between hemoglobin levels and quality of life in end-stage chronic kidney disease patients undergoing chronic hemodialysis at Hasan Sadikin Hospital 2021–2022. Indonesian Journal of Kidney and Hypertension, 1(1), 17–23. https://doi.org/10.32867/inakidney.v1i1.120

Hulu, I. P. S. (2025). Hubungan anemia dengan kualitas hidup pada pasien gagal ginjal kronik yang menjalani hemodialisa di RS Royal Prima Medan tahun 2024. Jurnal Ners, 9(2), 2856–2861. https://doi.org/10.31004/jn.v9i2.43959

Jelkmann, W. (2023). Anemia in chronic kidney disease: Pathophysiology and treatment. Biomedicines, 11(6), Article 1191. https://doi.org/10.3390/biomedicines12061191

Kementerian Kesehatan Republik Indonesia. (2024). Laporan tematik Survei Kesehatan Indonesia (SKI). Kementerian Kesehatan Republik Indonesia.

Kusumastuti, K. (2025). Periodic epidemiologi journal. Jurnal Berkala Epidemiologi, 13(3), 238–245. https://doi.org/10.20473/jbe.v13i32025.238

Parimala, L., & Sofia, M. (2021). Assessment of satisfaction level and quality of life among haemodialysis patient's on dialysis therapy. Medico-Legal Update, 21(1), 734–737. https://doi.org/10.37506/mlu.v21i1.2400

Parker, R. (2022). Alpha globin gene copy number is associated with prevalent chronic kidney disease and incident end-stage kidney disease among Black Americans. Journal of the American Society of Nephrology, 33(2), 213–224. https://doi.org/10.1681/ASN.2021050653

Portolés, J. (2021). Anemia in chronic kidney disease: From pathophysiology and current treatments, to future agents. Frontiers in Medicine, 8, Article 642296. https://doi.org/10.3389/fmed.2021.642296

Riskesdas. (2018). Laporan Riskesdas Sumut 2018. Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan.

Situmorang, P. R. (2025). Deteksi gen hemoglobin E (HbE) dengan metode elektroforesis pada pasien gagal ginjal kronik (GGK) di Rumah Sakit Santa Elisabeth Medan. Malahayati Nursing Journal, 7(11), 4684–4693. https://doi.org/10.33024/mnj.v7i11.22834

Sowersby, D. S. (2025). SURE gel electrophoresis: A method for improved detection and purification of dilute nucleic acid samples. Analytical Biochemistry, 684, Article 115373. https://doi.org/10.1016/j.ab.2023.115373

Syahrul, M. (2023). Pengaruh hemodialisis terhadap nilai hemoglobin pada pasien end stage disease di RS Stella Maris Makassar. Jurnal Ners, 7(1), 68–73. http://journal.universitaspahlawan.ac.id/index.php/ners

Thermo Fisher Scientific. (2024). Nucleic acid gel electrophoresis troubleshooting. https://www.thermofisher.com/id/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/na-electrophoresis-education/na-electrophoresis-troubleshooting.html

Widodo, S. (2023). Buku ajar metodologi penelitian. CV Science Techno Direct.

Wellem, I. (2021). Metodologi penelitian. Jurnal Pemerintahan Mandiri Lokal, 32(3), 167–186. https://doi.org/10.52152/801841

Wittmeier, P. (2022). Benchmark agarose gel electrophoresis to assess PCR product yield: Comparison with spectrophotometry, fluorometry and qPCR. BioTechniques, 72(4), 8–11. https://doi.org/10.2144/btn-2021-0094

World Health Organization. (2023). GBD 2023 Chronic Kidney Disease Collaborators. World Health Organization. https://doi.org/10.1016/S0140-6736(25)01853-7

Zhang, C. (2025). Emerging technologies and advanced strategies in hemoglobin defect screening. Journal of Clinical Medicine, 14(16), Article 5690. https://doi.org/10.3390/jcm14165690